 | Nanotech 2003 Vol. 1
Technical Proceedings of the 2003 Nanotechnology Conference and Trade Show, Volume 1
Chapter 2: Computational Biology |
| | Mathematical Modeling the Dynamics of Recombinant Endonuclease Production in Microsystems |
| Authors: | K. Ohtaguchi and K. Asami |
| Affilation: | Tokyo Institute of Technology, JP |
| Pages: | 17 - 20 |
| Keywords: | DNase I, Pichia pastoris, mathematical model, excretion system |
| Abstract: | A mathematical model for the dynamics of micro-scale production of recombinant human DNase I is formulated. In experiments extracellular production of human DNase I gene product from methylotrophic yeast Pichia pastoris GS115 has been achieved. A micro-scale production of recombinant human DNase I on a plug flow micro-scale reactor in steady-state was modeled mathematically. Simulation result suggests that the space time at which the maximum productivity of the gene product occurs is 37h. Micro-scale unit to separate endonuclease from cells was also designed using liquid-liquid extraction. Partition coefficient was considered in the models. |
 | View paper |
| ISBN: | 0-9728422-0-9 |
| Pages: | 560 |
| Hardcopy: | $125.00 |
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