Nanotech 2003 Vol. 1
Nanotech 2003 Vol. 1
Technical Proceedings of the 2003 Nanotechnology Conference and Trade Show, Volume 1

Computational Biology Chapter 2

Mathematical Modeling the Dynamics of Recombinant Endonuclease Production in Microsystems

Authors: K. Ohtaguchi and K. Asami

Affilation: Tokyo Institute of Technology, Japan

Pages: 17 - 20

Keywords: DNase I, Pichia pastoris, mathematical model, excretion system

Abstract:
A mathematical model for the dynamics of micro-scale production of recombinant human DNase I is formulated. In experiments extracellular production of human DNase I gene product from methylotrophic yeast Pichia pastoris GS115 has been achieved. A micro-scale production of recombinant human DNase I on a plug flow micro-scale reactor in steady-state was modeled mathematically. Simulation result suggests that the space time at which the maximum productivity of the gene product occurs is 37h. Micro-scale unit to separate endonuclease from cells was also designed using liquid-liquid extraction. Partition coefficient was considered in the models.

Mathematical Modeling the Dynamics of Recombinant Endonuclease Production in Microsystems

ISBN: 0-9728422-0-9
Pages: 560