Nano Science and Technology Institute
Nanotech 2003 Vol. 1
Nanotech 2003 Vol. 1
Technical Proceedings of the 2003 Nanotechnology Conference and Trade Show, Volume 1
 
Chapter 2: Computational Biology
 

Mathematical Modeling the Dynamics of Recombinant Endonuclease Production in Microsystems

Authors:K. Ohtaguchi and K. Asami
Affilation:Tokyo Institute of Technology, JP
Pages:17 - 20
Keywords:DNase I, Pichia pastoris, mathematical model, excretion system
Abstract:A mathematical model for the dynamics of micro-scale production of recombinant human DNase I is formulated. In experiments extracellular production of human DNase I gene product from methylotrophic yeast Pichia pastoris GS115 has been achieved. A micro-scale production of recombinant human DNase I on a plug flow micro-scale reactor in steady-state was modeled mathematically. Simulation result suggests that the space time at which the maximum productivity of the gene product occurs is 37h. Micro-scale unit to separate endonuclease from cells was also designed using liquid-liquid extraction. Partition coefficient was considered in the models.
Mathematical Modeling the Dynamics of Recombinant Endonuclease Production in MicrosystemsView PDF of paper
ISBN:0-9728422-0-9
Pages:560
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