Authors: M. Deshpande, K.B. Greiner, B.F. Romanowicz and J.R. Gilbert
Affilation: Microcosm Technologies, Inc., United States
Pages: 350 - 354
Keywords: CAD analysis, PCR well containment
Design analysis of PCR (Polymerase Chain Reaction) microwells are conducted to observe the contamination between neighboring wells on a PCR chip during the reaction. Thermal effects are incorporated to represent the temperature cycling characteristic of PCR. The analyses are intended to be predictive ñ and to be applied in the design of high throughput PCR devices. Parametric analyses were conducted to observe the effect of the allowable geometry variations on the contamination of the neighboring wells, and the effect of the variation in the diffusivities of the constituent chemical components. The analyses help in developing a set of curves that can be used to develop a model to represent the behavior of the PCR well. This model can then be implemented in system models for the entire device.