Authors: E. Kim, W. Kim, S-G Lee and S. Park
Affilation: Kyungpook National University, Korea
Pages: 242 - 245
Keywords: cytometric system, optical fiber, flow cell detection
In this experiment, we tested various optical fibers to select a suitable fiber for a effective flow cell detection. In order to align between micronozzle and optical fibers, a guide channel was fabricated by Si wafer etching with MEMS (Micro Electro-Mechanical System) technology. This fabricated system is advantageous due to its low cost and simple structure. This is possible because it replaces many optical lenses and expensive equipment with optical fibers. In result of cells detection by using various fiber modes in this system, for multi mode both input and output part, it was easy to align. The sensitivity of cell detection was not bad as other cases. The output voltage was as high as about 300ß∆, so we are going to use a light source which has relatively small output power. Therefore, it was suitable for minimization. By injecting various cells, we were able to detect cells. The reason for the various voltage characteristics is the difference of light permeability for each cell which is made up of specific cellulose and cell walls. In addition, we were able to compare the relative size of the injected cells.