Nano Science and Technology Institute
MSM 2000
MSM 2000
Technical Proceedings of the 2000 International Conference on Modeling and Simulation of Microsystems
 
Chapter 15: Applications: Micropumps, Heaters and Coolers
 

Simulation and Experimental Verification of Micro Polymerase Chain Reaction Chip

Authors:Y-C. Lin, C-C. Yang, M-Y. Hwang and Y-T. Chang
Affilation:Cheng Kung University, TW
Pages:648 - 651
Keywords:PCR, DNA, FEA
Abstract:This study used finite element analysis to simulate the temperature characteristics of a micro polymerase chain reaction (PCR) chip. The micro-PCR chip was fabricated on a silicon wafer and Pyrex glass using photolithography, wet etching, and anodic bonding methods. The main goal of this study was to analyze the temperature uniformity and distribution of the micro-PCR chip, the temperature distribution of the DNA sample, and the transient temperature difference between the heater and DNA sample. The finite element analysis results were also confirmed by one-dimensional theoretic analysis. The simulation results were used to improve the thermal cycling time of a rapid micro-PCR system, consisting of a rapid thermal cycling system and a micro-PCR chip. The improved thermal cycles of the rapid mPCR system were verified using serum samples from patients with chronic hepatitis C. The hepatitis C virus (HCV) amplicon of the rapid mPCR system was analyzed by slab gel electrophoresis with DNA marker separation in parallel.
Simulation and Experimental Verification of Micro Polymerase Chain Reaction ChipView PDF of paper
ISBN:0-9666135-7-0
Pages:741
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