Labeling and Imaging of Human Mesenchymal Stem Cells with Quantum Dot Bioconjugates during Proliferation and Osteogenic Differentiation in Long Term

May 12, 2005 By: B. Shah, P. Clark, M. Stroscio and J. Mao, University of Illinois at Chicago

Quantum dots (QDs) are semiconductor nanocrystals that serve as promising alternatives to organic dyes for cell labeling. Because of their unique spectral, physical and chemical properties, QDs are useful for concurrently monitoring several intercellular and intracellular interactions in live normal cells and cancer cells over periods ranging from less than a second to over several days (several divisions of cells).

Here, peptide CGGGRGD is immobilized on CdSe-ZnS QDs coated with carboxyl groups by cross linking with amine groups. These conjugates are directed by the peptide to bind with selected integrins on the membrane of human Mesenchymal stem cells. Upon overnight incubation with optimal concentration, QDs effectively labeled all the cells. Here, we report long-term labeling of human bone-marrowderived mesenchymal stem cells (hMSCs) with RGDconjugated quantum dots (QDs) during self replication and differentiation into osteogenic cell lineages.

Nanotech 2005 Conference Proceedings

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