Site-Directed Chemical Modification of Phage Particles
L. Saleh, K.A. Noren, C.J. Noren
New England Biolabs, US
Keywords: phage, chemical modification, selenocysteine, medical imaging, semisynthesis
Abstract:The ability to chemically modify the surface of bacteriophage bypasses the functional limitations imposed by the standard biosynthetically-incorporated amino acids that comprise the phage coat. Appended functionalities can include fluorescent or other reporter groups, inorganic materials, cytotoxic agents, and pharmacophores. Applications include a) incorporating the modification in the context of a displayed random peptide library prior to panning as a route to chimeric semisynthetic peptide ligands, b) the use of phage as a template for construction of novel nanomaterials, c) direct mechanical manipulation of phage, d) the use of phage particles as medical imaging reagents, and e) catalysis-based screening for novel enzyme activities.