Effects of Crosslinking Condition on Mechanical and Biological Properties of Biomimetic Fibrous Composite Material for Arterial Medial Equivalent
K. Madhavan, D. Belchenko, W. Tan
University of Colorado at Boulder, US
Keywords: collagen, genipin, viscoelasticity, biocompatibility, smooth muscle cell
Abstract:This study involves the mechanical and biological characterization of crosslinked collagen-based scaffolds for arterial medial-equivalent. These scaffolds are made from a mixture of collagen, chitosan and elastin and crosslinked with genipin. The method of optimization of mechanical and biological properties involves the variation of concentration of components (collagen & chitosan) and crosslinker (genipin). Varying the concentration improves the biomimetic mechanical properties. Even though smooth muscle cells and endothelial cells exhibit high biocompatibility to genipin-crosslinked scaffolds, varying the genipin concentration affects the cell ingrowth in these scaffolds. By striking a balance between the mechanical and biological properties, we are able to design a suitable material.