Microfluidic Platforms with Micro- and Nanopore Membranes and in situ Formation of Lipid Bilayer in the Microfluidic Platform
J. Choi, N. Kim, S. Park
Louisiana State University, US
Keywords: lipid bilayer, microfluidic, micro- and nanopore membrane
Abstract:Artificial lipid bilayer membranes have come into the spotlight as topics of fundamental properties of biomaterials, targets for drug discovery and safety screening, and analytic platforms for characterization and orientation discrimination of polynucleotide molecules. Lipid bilayer membranes are typically formed across micro- and macroscale pores as support materials that separate two biological (inter- and extracelluar) environments. However, integration of lipid bilayer membranes into microfluidic platforms, which will allow for minimal use of reagents, decrease of analysis time, and portability of the fabricated devices, has seldom been carried out. Therefore, it is needed to develop a stable and flexible method for integrated microfluidic system with lipid bilayer membranes that have access to both sides of the membrane. In this study, we will show in situ formation of a lipid bilayer within microfluidic platform containing the perforated polymer membrane with the method used by Suzuki et al., which will be confirmed by measuring transitional trans-membrane current and impedance.