Development of a Flow-cell System to Quantify Analyte-receptor Binding Kinetics Utilizing Guided-mode Resonance Sensors
D. Wawro, S. Zimmerman, Y. Ding, R. Magnusson, E. Moss, J. Michalik
Resonant Sensors Incorporated, US
Keywords: flow-cell system, guided-mode resoance, biosensor
Abstract:A novel flow-cell system integrated with a guided-mode resonance sensor element array is presented. The association/disassociation binding cycle is monitored for protein-protein and protein-cell interactions. The real-time binding interactions of the antibody IgG and protein-A (S. aureus) have been measured and will be presented. The sensor elements are fabricated in polymer materials by a contact printing method in which a grating master is used as a replication template. The resulting copied grating structure is coated with a thin dielectric layer to create the final sensor device. A microfluidic flow-cell chamber made from poly(dimethylsiloxane) through a molding process is bonded to the sensor element. The results obtained show that the microfluidic flow-cell system can be used to monitor the entire association/dissociation-binding cycle, which is not possible with the static system. Current research is in progress aiming to expand the microfluidic flow-cell chamber into a highly-parallel and automated system to allow for multiple types of biological assays to be conducted simultaneously using the sensor method described. Engineered systems of this type may prove effective in characterizing many key biomolecular and cell binding reactions under conditions of dynamic liquid flow. Their development and verification is the chief aim of this research.