Assembly of hydroxylapatite nanoparticles on hydroxylapatite-binding phage selected from a landscape phage library

B. Cao, S. Modali, G. Abbineni, P. Jayanna, V.A. Petrenko, C. Mao
University of Oklahoma, US

Keywords: landscape phage display, bone mineral, assembly

Abstract:

We selected peptides that can bind to hydroxyapatite [HAP, (Ca5(PO4)3OH)2] by using landscape phage display technique. We found that the HAP-binding peptides share some motifs with bone-specific proteins such as collagen and osteocalcin. We interacted the HAP-binding phage with preformed HAP nanoparticles and found that the HAP nanoparticles can be assembled into an array along the phage body. The HAP-coated phage may serve as a building block for further assembly into a higher-order structure.