Cell targeting and nanoscale drug delivery using purified virus envelope proteins
J. Wang, A.A. Kolokoltsov, R.A. Davey
University of Texas Medical Branch, US
Keywords: drug delivery, cell targeting, membrane penetration
Abstract:The delivery of drugs and other small molecules, like siRNA, into cells and tissues is generally an inefficient, random process, where drugs are flooded through the circulation and some finds its way to the target. Previously, we reported our success in the development of a nanoparticle coating technology where membranes were stripped from virus particles and coated over the surface of nanoparticles by simple extrusion (Nano Lett. 2006;6:2414-21). The nanoparticles not only gained the receptor specificity of the virus but were released into the cell cytoplasm. This offered a new method to deliver drugs. However, the virus material used was only partially purified and still contained unwanted virus proteins and genetic material. In our continuing efforts to develop this system we have defined better ways to eliminate the unwanted material while increasing specific activity. We will present our work describing the best method of purifying the functional envelope proteins and how the charge properties of the nanoparticle itself can play a major role in envelope protein coating efficiency and interaction with cells.