Analysis of different protein functionalization for polymeric electrospun nanofibers
A. Polini, S. Pagliara, R. Stabile, R. Cingolani, D. Pisignano
National Nanotechnology Laboratory of INFM-CNR, IT
Keywords: electrospinning, nanofibers, functionalization
Abstract:Here we investigate the crucial step of protein functionalization for prototype polymer nanofibers. We compare different coating approaches for a target adhesion protein, including both physisorption and covalent binding methods relying on different functional linkers. Scanning electron microscopy, wettability measurements, Fourier-Transform infrared spectroscopy, protein quantification and cytotoxic assays are performed.