Cluster-assembled nanostructured TiO2 coating as novel biomaterial for hematopoietic cell adhesion
E. Barborini, R. Carbone, A. Zanardi, M. De Marni, S. Venturini, M. Leccardi, D. Bandiera
Tethis srl, IT
Keywords: nanomaterials, titanium oxide, coating, cell adhesion, device
Abstract:We recently introduced cluster-assembled nanostructured TiO2 (ns-TiO2) produced by supersonic cluster beam deposition (SCBD), as a novel coating showing high specific surface due to nanoscale roughness and porosity, good adhesion on glass, optical transparency, absence of fluorescence background signal, and biocompatibility. Here we show the results on adhesive properties respect to hematopoietic human cells, where we surprisingly discovered, that ns-TiO2 strongly enhances their adhesion, even in condition of microfluidic shear stress. To this purpose, PDMS slides with microfluidic channels have been clamped on ns-TiO2 coated glass slides and, as control, on uncoated glass slides. Hematopoietic U937 cell suspension has been loaded into each channel and incubated for 2’ at 37°C. After cell fixation and extensive washing, we have performed DAPI staining to visualize the nuclei through fluorescence microscopy. Around 80% of cells are recovered at the end of the experimental protocol on coated slides, while less than 10% are present on the uncoated ones. Regarding the deposition method, SCBD allows room-temperature direct deposition on any kind of substrate and hard-mask patterning, avoiding photolithographic processes. These features provide compatibility with microfabrication and miniaturization, disclosing the feasibility of producing nano-coated microdevices and biochips for fluorescence based analytical assays.