Design, characterization and in vitro cytotoxicity of biocompatible Pt(acac)2-TiO2 nanoparticles
T. Lopez, R.D. Gonzalez, M. Alvarez, E. Ortiz-Islas
Instituto Nacional de Neurología y Neurocirugía, MX
Keywords: nanoparticles, cancer, DNA adducts,platinum acetylacetonate
Abstract:We designed Pt(acac)2 -TiO2 and Pt(acac)2-SiO2 nanostructured materials with the purpose of obtaining novel materials with antitumoral activity to be used as an alternative in the treatment of certain tumors. The biocatalysts were prepared by the sol-gel route using the complex Pt(acac)2 (1%). The sol-gel parameters were controlled in order to obtain high surface area, high platinum dispersion and particles in the nano-size range. TEM, FTIR, N2 adsorption and XPS characterization studies of the samples were carried out. The method of synthesis enabled us to obtain a high dispersion of the metal. In order to investigate interactions between the biocatalyst and DNA, agarose gel electrophoresis was performed, and we observed the formation of DNA adducts with all samples. After 45 minutes of interaction, Pt(acac)2-TiO2 completely degraded the DNA, more rapidly than cisplatin (120 minutes). The biocatalysts were tested in C6 animal brain models, placing the biocatalyst directly in the tumor and a significant decrease in size was observed. These results demonstrate that using a minimum dose of platinum complexes,can be effectively used in the treatment of localized tumors.