Celecoxib loaded polymersomes for controlled drug delivery and anticancer therapy
H. Vishwasrao, J. Eley, M. May, J. Bigelow
Idaho State University, US
Keywords: polymersomes, pancreatic cancer, celecoxib, cytotoxicity
Abstract:Polymersomes are nano-vesicles made up of amphiphilic block copolymers. They capable of carrying a much higher payload of drugs than liposomes and thereby achieve the two main goals of controlled drug delivery namely, spatial placement and temporal delivery of a drug. Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and virtually incurable forms of cancer. Recent studies have revealed that cyclooxygenase-2 (COX-2) enzyme inhibitor drugs such as Celecoxib and Rofecoxib have shown considerable growth inhibitory activity against it . Therefore the main aim of this study was (i).to determine the cytotoxic potential of celecoxib on panc10.05 PDAC cell lines, (ii). To prepare and characterize polymersomes loaded with Celecoxib for their particle size, entrapment efficiency and drug loading capacity and (iii). To determine the cytotoxic efficacy of the drug loaded polymersomes on panc10.05 cell lines. Our results suggest that celecoxib has a very narrow therapeutic index as an anticancer agent, with no significant cytotoxicity being shown below 80µM concentration. However when loaded in polymersomes, even the non-cytotoxic doses exhibit more than 50% mortality. The formulation is also able to provide a sustained effect for a period of atleast72 hours thereby exhibiting excellent controlled drug delivery potential.