Role of Ultrasound in Anti-Cancer Drug Delivery Loaded on Microspheres
M.M. Mohamed, M.A. El belbese, M.A. Kotb, N.M. Fikry, H.S. Ramadan
Alexandria University, EG
Keywords: drug delivery, cancer, cisplatin chitosan microparticles
Abstract:Targeting is a new therapeutic tool for malignant tumor as a result of combining nanotechnology with chemotherapeutics. Ultrasound has been shown to enhance degradation and drug delivery from biodegradable and non biodegradable polymeric devices. If a microsphere is partially filled with an entrapped drug substance, it is then able to transport the drug through blood vessels and release its load upon being triggered by an ultrasound pulse, which cracks the shell. The aim of our study is to investigate the role of ultrasound in anti-cancer drug delivery loaded on microspheres The cisplatin chitosan microparticles was prepared by the membrane emulsification technique. A total of 80 male Swiss albino mice weighting (20-25 g) received subcutaneous injections of 2x10 6 (Ehrlich ascites carcinoma) cells mammary in origin. A week later, the tumor bearing mice was divided into four main groups: group of 20 mice serves as a control untreated group, group of 20 mice which were injected with cisplatin only, group of 20 mice which were injected with blank chitosan microspheres, and a group of 20 mice which were injected with cisplatin chitosan microspheres. Each group was divided into two subgroups each of 10 mice, where one group was treated with ultrasonic waves and the other subgroup received no treatment with ultrasonic waves. Calculations were made 25 days after treatment in order to compare the densities, volumes, inhibition ratios and growth curves of the tumors in each group. Mice in each group were sacrificed randomly to collect tumor tissues and other organs for electron microscopy and pathological examination. Results obtained indicate that tumor growth delayed 4 t0 6 days by combined treatment of cisplatin chitosan microparticles and ultrasound than that with cisplatin chitosan microparticles alone. Ultrastructure investigations of tumor cells showed a severe damage in cytoplasmic organells and cytoplasmic vacuoles.