In Vivo Biodistribution of a Multistage Nanodelivery System for Therapeutic Applications and Medical Imaging
E. Tasciotti, X. Liu, R. Serda, C. Chiappini, B. Godin-Vilentchouk, R. Bhavane, M. Ferrari
Univeristy of Texas Health Science Center - Houston, US
Keywords: drug delivery, multi-stage, porous silicon, biodistribution, nanodelivery, therapeutics
Abstract:The ability to deliver therapeutic compounds specifically to diseased sites is crucial for effectively treating human illnesses. Nanotechnology is emerging as a tool for resolving challenges in the delivery of poorly administrable drugs. We hypothesized, engineered, developed and tested multi-stage system for systemic delivery (MSDS) designed to successfully overcome sequential biological barriers. We developed a MSDS using biodegradable, biocompatible silicon particles optimally tailored to travel into the blood flow, avoid RES, marginate and adhere to tumor vasculature. These first stage carriers (FSCs) contain nanopores that can load, carry, release over time, and deliver multiple types of second stage nanoparticles. The nanoparticles are optimized to reach the tumor environment and release their therapeutic payload into target cells. It is possible to conjugate the FSCs with fluorescent and radiolabeled imaging molecules. Fluorescent and magnetic SSNs can be loaded into the pores of the FSCs. Once assembled, the resulting MSDS can be imaged from its administration to its final localization into the body. We monitored the biodistribution of the FSCs and characterized their biocompatibility in the different tissues. These studies provide first time evidence of silicon nanoporous particles use as effective carriers for the simultaneous delivery of different nanotherapeutics in vivo.