NSTI Nanotech 2009

Viral Gene Therapy for Expression of Transporter Gene in Pancreatic Tumor Cells: A Novel Approach

A. Paul, A. Kulamarva, M. Malhotra, S. Prakash
McGill University, CA

Keywords: gene therapy, transgene, pancreatic cells, baculovirus

Abstract:

Recent advances in molecular biology and therapeutic delivery systems have brought about the development of novel approaches to treat pancreatic cancer including gene therapy, immunotherapy, and biological therapy. Here we report a recombinant baculoviral gene delivery system that can efficiently transport GFP transgene to human pancreatic cells because of its rod shaped nanostructure with gp64 cell membrane glycoprotein. The prepared high titer viruses were then allowed to transduce the growing pancreatic cells for 8hrs, after which, it was replaced by normal cell media. Just 24hr post transduction the cells were checked under fluorescence microscope followed by flow cytometry. Cytotoxicity test of the cells towards different viral titers was done using cell proliferation assay and optimization of amount of viral particles needed for maximum transduction efficiency was determined. The viruses showed no cytotoxicity towards the cells. There was a large enhancement of transduction efficiency in the cells treated with sodium butyrate, the deacetylase inhibitor, in comparison to the untreated. Thus we concluded that baculovirus can be a biologically safe potential gene transfer tool for pancreatic carcinoma not only for studying the function of foreign genes but also for development of novel gene delivery strategies targeting different cancer cell types.
 
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