NSTI Nanotech 2009

Cracking the glass ceiling with suspended microarrays: Achieving true diagnostic quality assays

B.P. Hanley
KonnectWorld, Inc., US

Keywords: suspended microarray variance microbeads luminex accuracy

Abstract:

There are a number of intractable sources of variance in suspended microarrays run through flow cytometry equipment that prevent achieving diagnostic quality. Highly unpredictable sets of microbeads can appear that were sourced from unidentifiable samples. Flow cytometer sensitivity can vary in unexpected ways. Microbead sizes can vary considerably more than specifications would lead us to believe. Agglutination can potentially occur, and these assays are statistical case studies in generation of bell curve histograms of bead counts. All of these problems are addressable by a combination of techniques that do not require changes to the flow cytometry instruments.
 
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