Development of a biochip sensor on the basis of high ordered porous membranes (SmartMembranes) as diagnostic systems for DNA and protein species as well as for prions
P. Goering, M. Lelonek, R.B. Wehrspohn
Martin-Luther-University Halle-Wittenberg, DE
Keywords: macro porous silicon, nano porous alumina, DNA sensor, prion
Abstract:Biosensors are sensing elements which are characterized through the coupling of a biological detection element (receptor) with a signal converter (transducer). They distinguish their function by a high selectivity for biochemical analytes. Biochips as well as DNA chips enable the detection of proteins, enzymes and DNA sequences by interacting with specific modified surfaces. Further advancement of those biochip sensors can be found in micro and nano array systems. They are composed of micro and nano po-rous materials with a high ordered and open pore structure. The company SmartMembranes is planning to develop biochips on the basis of micro and nano arrays with a high ordered pore structure as well as the manufacturing of marketable components for biochip sensors. These will be integrated in complete measuring systems for DNA, protein and prion analysis. The basis for the suggested innovative biosensors is the optimized electro chemical etching processes for the fabrication of macro porous silicon (Si) and nano porous alumina (Al2O3) - further developed by the Fraunhofer Institute for Mechanics of Materials in cooperation with the Martin-Luther-University Halle-Wittenberg and the Max-Plank-Institute for Micro-structure Physics Halle. These processes enable a series production of high ordered porous membranes with strongly parallel pore arrangement. The technology allows the preparation of micro and nano pore arrays on substrate areas up to 6 inch with pore densities from 10e7/in2 up to max. 10e11/in2. In contrast to customary biochips those pore arrays provide the advantage of a lot larger surface which can be used for all kinds of surface reactions. Therefore the measuring sensi-tivity as well as the measuring speed can be strongly enhanced. The regularity of an ordered pore array also opens the possibility to exactly address the ar-eas of the biochip and hence to detect different analytes simultaneously.