Reactive Nitrogen Species Scavenging Properties of Cerium Oxide Nanoparticles

J.M. Dowding, W. Song, A. Karakoti, S. Seal, E. Bossy-Wetzel, W.T. Self
University Central Florida, US

Keywords: ceria nanoparticles, reactive nitrogen species, antioxidants

Abstract:

In this study, we investigated the ONOO- and NO. scavenging potential of CeO2 nanoparticles. APF assays demonstrate the ability of CeO2 NPs to interact with ONOO- and prevent APF oxidation in a dose dependant manner. Spectrophometric results indicate that CeO2 NPs directly interact with ONOO-. Results from the hemoglobin assay showed that as NO• was released, CeO2 NPs did not prevent NO• from reacting with HbO2. When cells were pre-treated for 72 h with CeO2 NPs, the cultures showed protection from nitrative stress.