Mechanism of Cellular Uptake of Carboxyfluorescein Modified Ceria Nanoparticles in Human Keratinocyte cells
S. Singh, A. Kumar, A.S. Karakoti, S. Seal, W.T. Self
University of Central Florida, US
Keywords: nanoceria, cellular uptake, endocytosis
Abstract:Here we have performed a systematic study for cellular uptake of fluorescent molecule (carboxyfluorescein) conjugated ceria nanoparticles (CeO2 NPs). Cellular uptake of CeO2 NPs, was monitored by fluorescent microscopy for varying exposure times and concentrations. Well established inhibitors of endocytosis were used to determine the mechanism of uptake. A kinetic study was performed where cells were exposed to NPs for 1h, 3h, 6h and 24h. No significant increase in fluorescence was recorded after 6 hours incubation (Figure shown underneath), suggesting the uptake reached equilibrium within 6 hours. Preliminary results suggest CeO2 NPs internalization is energy dependent as no significant uptake was recorded after the treatment of NaN3 and 2-deoxy-D-glucose which is known to disrupt ATP production in cells . Further experiments are underway to give more insight to cellular uptake and sub-cellular localization of CeO2 NPs.