Rapid prototyping of microfluidic components for decision-tree based iterative pathogen detection
P. Nath, G.R. Goddard, B.A. Branch, O.C. Marina, A.M. Lillo, A. Deshpande, J.M. Dunbar, S.W. Graves
Los Alamos National Lab, US
Keywords: microfluidics, lab-on-a-chip
Abstract:We are developing an autonomous detection system that rapidly detects and identifies pathogens using decision-tree based iterative assays; i.e. to first test for a general panel of pathogens followed by increasingly specific tests to precisely identify the pathogen or critical properties (e.g. antibiotic resistance). Field based micromanipulations and micro-sphere based arrays are utilized to develop the programmable sample preparation and analysis steps. Acoustic, magnetic and dielectrophoretic (DEP) fields are utilized for sample preparation (e.g. cell-lysis, mixing, trapping and washing), where as, bead based immunoassays and Multiplex Oligo Ligation - Polymerase Chain Reactions (MOL-PCR) are utilized for analysis. Rapid prototypes of the required microfluidic components enabling mixing, trapping and PCR are developed using off-the-self materials (e.g. acrylic sheets, adhesive transfer tapes) and a CO2 laser based engraving tool. In this presentation, the description of our rapid prototyping process and the ability of the prototypes to perform individual steps (e.g. mixing, trapping, PCR) for decision-tree based iterative assays will be discussed in further details.