Method of LANTCET for cancer diagnostics and treatment at cell level
E.Y. Hleb, I.A. Zastinskaya, I.I. Ilyukova, I.N. Semenenya, J.H. Hafner, E.Y. Hanna, J.N. Myers, S.A. Zhdanok, D.O. Lapotko
A. V. Lykov Heat & Mass Transfer Institute, BY
Keywords: cancer, nanoparticles, laser, bubble
Abstract:We have developed the method of LANTCET (laser-activated nano-thermolysis as a cell elimination technology) for selective detection and destruction of individual tumor cells by generating intracellular photothermal bubbles around the clusters of gold nanoparticles. Bare nanoparticles and their conjugates with the C225 tumor-specific monoclonal antibodies were applied in vitro to the C225-positive squamous carcinoma cells and in vivo to an experimental tumor in a rat to form intracellular clusters of nanoparticles. Single 10 ns laser pulses generated intracellular photothermal micro-bubbles at a near-infrared and visible wavelengths. The cells with the clusters have yielded almost 100-fold decrease in laser fluence thresholds for bubble generation and cell damage relatively to those for the cells without clusters. Cell damage had a mechanical origin and single cell selectivity. Three LANTCET processes (cell detection, damage, and optical guidance) were realized as a microsecond sequence and with the one device.