Biomimetic thin fibrin network as a scaffold for endothelial cells
T. Riedel, E. Brynda, J. Chlupac, E. Filova, L. Bacakova, J.E. Dyr
Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, v.v.i., CZ
Keywords: fibrin, nanostructures, cell seeding, vessel prosthesis
Abstract:Knitted polymer vascular prostheses are commonly used for the replacement of damaged vascular vessels. However, small diameters synthetic grafts displays high failure rates due to thrombus formation and perianastomotic hyperplasia. In this study, artificial fibrin nanostructures, prepared by novel technique based on the catalytic activity of surface-bound thrombin on fibrinogen solution, and assemblies of extracellular matrix proteins were prepared on knitted polyethylene terephtalate vascular prostheses previously impregnated with copolymer poly(L–lactide–glycolide) or the terpolymer poly(L–lactide–glycolide–caprolactone). Although the modification of prosthesis decreased the initial adhesion of endothelial cells, it increased the proliferation of endothelial cells. Best proliferation was found on surfaces coated with fibrin network and on fibrin network modified with fibronectin, where almost confluent layer of cells were formed after 7 days. The developed coating techniques make it possible to encourage the endothelization vascular prosthesis lumen while preserving the free space in the vessel wall for potential capillary vascularization.