Oxidase and Dehydrogenase-based Glucose Bioanodes for Starch Biofuel Cell Development
M-J Yen, J-Y Wang, K-C Ho, L-C Chen
Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, TW
Keywords: biofuel cell, starch, glucose, oxidase, dehydrogenase
Abstract:This work reports two glucose bioanode systems for starch biofuel cell development based on glucose oxidase and glucose dehydrogenase, respectively. The Starch biofuel cell is a novel topic and can turn starch into electrical energy and the maximum power density is 30 microWcm-2 in the 1 wt% starch. However, taking account of the optimal system for bienzyme kinetics, mediator immobilization is an essential issue. For this purpose, methylene blue, which is a catalyst for NADH, can be used as a dehydrogenase-based bioanode. The efficiencies of NADH electrocatalysis at the PMB and PMB/MWCNT electrodes are evaluated by amperometric NADH sensing without GDH addition. The detection is demonstrated by flow injection analysis (FIA) system and the sensitivities from the linear calibration curves are ca. 13.9 and 26.3 microA mM-1cm-2 for PMB and PMB/MWCNT, respectively. This result discloses that the nanocomposite of PMB and MWCNT can serve as a highly efficient electrocatalyst for NADH oxidation. Besides, this improvement of NADH electrocatalysis also indicates that the efficiency of starch biofuel cell could be improved by applying PMB/MWCNT-modified bioanode. In spite of the preliminary experiments for bioanode, the oxidase and dehydrogenase-based starch biofuel cell systems will be described in this symposium.