2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Long-Circulating Micelle-like DNA-encapsulating Nanoparticles for Systemic Gene Delivery

Y.T. Ko, W.C.A. Kale, V.P. Torchilin
Northeastern University, US

Keywords:
In vivo gene delivery, systemic gene delivery, long-circulating nanoparticle

Abstract:
This study describes a novel nanoparticle for systemic gene delivery using phospholipids-polyethylenimine (PLPEI) conjugate in which polyethylenimine (PEI) is conjugated to the distal end of phospholipids alkyl chain. The PLPEI along with PC, cholesterol, PEG-PE is mixed with plasmid DNA to form nano-sized complexes with the structure of PEI/DNA polyplex core and phospholipids monolayer envelope. The final construct is sterically stabilized micelle-like ‘hard core’ nanoparticle (MLP) with PEI/DNA polyplex core and lipid monolayer envelope. MLP showed prolonged circulation time along with an insignificant accumulation at RES sites, providing a promising tool for systemic gene therapy.


Nanotech 2008 Conference Program Abstract