Long-Circulating Micelle-like DNA-encapsulating Nanoparticles for Systemic Gene Delivery
Y.T. Ko, W.C.A. Kale, V.P. Torchilin
Northeastern University, US
In vivo gene delivery, systemic gene delivery, long-circulating nanoparticle
This study describes a novel nanoparticle for systemic gene delivery using phospholipids-polyethylenimine (PLPEI) conjugate in which polyethylenimine (PEI) is conjugated to the distal end of phospholipids alkyl chain. The PLPEI along with PC, cholesterol, PEG-PE is mixed with plasmid DNA to form nano-sized complexes with the structure of PEI/DNA polyplex core and phospholipids monolayer envelope. The final construct is sterically stabilized micelle-like ‘hard core’ nanoparticle (MLP) with PEI/DNA polyplex core and lipid monolayer envelope. MLP showed prolonged circulation time along with an insignificant accumulation at RES sites, providing a promising tool for systemic gene therapy.
Nanotech 2008 Conference Program Abstract