Nanoscale Confinement of Antibodies towards biosensor interfaces with enhanced sensitivity
S. Krishnamoorthy, M. Himmelhaus
Fujirebio, Inc., JP
nanopartterning, biosensor, antibody
We present our latest results on nanopatterning of antibodies. In earlier work we had found that antibody immobilization onto functional nanopatches created in a protein-resistant matrix leads to an increase of antibody (Ab) activity. In contrast, however, when the matrix is not protein-resistant, no improvement in Ab activity had been observed. Our results suggested a better orientation and denser Ab packing on the protein-resistant patterns. Now we achieved direct experimental evidence for confinement and improved orientation of antibodies when immobilized on nanopatches embedded in a PEG matrix by atomic force microscopy. The images clearly show that the Ab/Ag complexes are confined to the functional nanopatches and that the total feature heights correspond to the Ab/Ag sandwich.
Nanotech 2008 Conference Program Abstract