2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

Nanoscale Confinement of Antibodies towards biosensor interfaces with enhanced sensitivity

S. Krishnamoorthy, M. Himmelhaus
Fujirebio, Inc., JP

Keywords:
nanopartterning, biosensor, antibody

Abstract:
We present our latest results on nanopatterning of antibodies. In earlier work we had found that antibody immobilization onto functional nanopatches created in a protein-resistant matrix leads to an increase of antibody (Ab) activity. In contrast, however, when the matrix is not protein-resistant, no improvement in Ab activity had been observed. Our results suggested a better orientation and denser Ab packing on the protein-resistant patterns. Now we achieved direct experimental evidence for confinement and improved orientation of antibodies when immobilized on nanopatches embedded in a PEG matrix by atomic force microscopy. The images clearly show that the Ab/Ag complexes are confined to the functional nanopatches and that the total feature heights correspond to the Ab/Ag sandwich.


Nanotech 2008 Conference Program Abstract