2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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Clean Technology 2008

Targeting and Delivery of siRNA using Nanoparticles Fabricated via the PRINT Process

D.A. Canelas, X. Gao, S. Tian, P.A. Ropp, J.M. DeSimone
University of North Carolina at Chapel Hill, US

Keywords:
nanoparticles, siRNA, PRINT

Abstract:
The objective of our work is to exploit the Particle Replication in Non-wetting Templates (PRINT) process to prepare and deliver uniform polymer nanoparticles containing sensitive biological cargo such as siRNA or antisense oligonucleotides. The PRINT process effectively yields particles with a narrow size and shape distribution, and the biological cargo is directly incorporated during the particle fabrication process. Previous studies in our lab have determined the biodistribution of blank control particles in mice and monitored the ability of HeLa cells to internalize non-spherical, monodisperse PRINT hydrogel particles based on differences in size, shape, and surface charge. Particles for the studies reported herein are constructed of lightly crosslinked poly(ethylene glycol) (PEG) or poly(vinyl pyrrolidone) (PVP). The crosslinking groups contain disulfide or acetal functionalities known to cleave under endosomal conditions. Initial studies described in this paper focus on using these particles to facilitate cargo delivery using luciferase knockdown quantification and cell viability assays in a luciferase-transfected HeLa reporter cell line. Efficient uptake of the particles by the cells was confirmed using fluorescein-labeled particles and FACS. Ongoing in vitro experiments aim to prepare particles that will degrade and release cargo upon endosomal uptake.


Nanotech 2008 Conference Program Abstract