2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

High Fidelity Hompolymer Sequencing

S. Goel, P. Griffin, R.W. Davis, M. Ronaghi
Stanford University, US

Keywords:
genome sequencing, homopolymer, bioluminescence, pyrosequencing

Abstract:
The main requirement of the next generation genome sequencing platform is achieving the goal of one thousand dollars per human genome with 99.99% accuracy. To accomplish this goal, a highly integrated platform with the new implementation of a robust sequencing technology and an extremely sensitive imaging system with proven reputation are needed. Although many emerging sequencing technologies address the issue of cost reduction and give a strategy to achieve the goal of thousand dollars per genome, still lot of work needs to be carried out to attain not more than one error in every 10,000 bases. The main source of error is due to incomplete incorporation of homopolymeric regions in the genome during sequencing. Here we present a strategy based on Pyrosequencing to achieve accurate and high fidelity incorporation of upto 10 bases long homopolymeric regions. Pyrosequencing relies on the bioluminometric real-time detection of inorganic pyrophosphate released on successful incorporation of nucleotides during DNA synthesis. The system includes a high-density picotiterplate carrying DNA beads, a fluid delivery system controlling pyrosequencing reagents and a sensitive photomultiplier tube.


Nanotech 2008 Conference Program Abstract