2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Developing superparamagnetic iron oxide nanoparticles as tumor-specific magnetic resonance imaging (MRI) contrast agents

R.M. Sawant, E. Gultepe, D. Nagesha, S. Sridhar, V.P. Torchilin
Northeastern University, US

Keywords:
MRI, iron oxide nanoparticles, polymeric micelles, targeted, cancer

Abstract:
Superparamagnetic iron oxide nanoparticles (SPION) have received increased interest due to their characteristic small size ca 4 -10 nm and excellent T2-type MRI contrast properties. However, uncoated-“plain” SPION have a tendency to aggregate and thus are not stable at normal physiological conditions. In this work we load the SPION in polymeric polyethylene glycol phosphatidyl ethanolamine (PEG-PE) micelles and additionally surface-modify with anticancer anti-nucleosome antibody 2C5. (mAb 2C5). SPION-loaded PEG-PE micelles were stable with the size ranging from 20 to 40 nm. The conjugation of mAb 2C5 was gentle and there was almost no loss in activity of the antibody after conjugation. SPION-loaded mAb 2C5 immunomicelles were able to recognize and bind with human breast cancer MCF-7 cells in vitro significantly higher when compared to SPION-loaded “plain” micelles or SPION -loaded “non-specific” bovine IgG immunomicelles. SPION-loaded mAb 2C5-immunomicelles were thus able to bring higher quantity of SPION to these cells, and have a potential to serve as an MRI contrast agent with improved T2 contrast signal for better tumor imaging.


Nanotech 2008 Conference Program Abstract