2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Directed Self-Assembly of Bio-Derivatized Nanoparticles into Micron Size Biosensor Devices

M.J. Heller, D. Dehlinger, S. Esener, A. Hsiao
University of California, San Diego, US

Keywords:
self-assembly, nanoparticles, nanofabrication, biosensors

Abstract:
An electronic array has been used to carry out the directed self-assembly of higher order 3D structures from Biotin/Streptavidin and DNA derivatized nanoparticles. Structures with more than fifty layers of alternating biotin and streptavidin and DNA nanoparticles have now been fabricated using a microarray system. The directed nanoparticle layering process takes about one minute per layer; 10-20 seconds for addressing and binding nanoparticles and 40 seconds for washing. The final multilayered 3-D structures are about two microns in thickness and 50 microns in diameter. Work is now focused on assembling “micron size” bio/chemsensor devices from luminescent, fluorescent and enzyme derivatized nanoparticles. The structure for a nanolayered glucose sensor device includes a base layer of biotin/streptavidin nanoparticles, a layer of glucose oxidase derivatized nanoparticles, a layer of peroxidase derivatized nanoparticles, a layer of quantum dots, and a final layer of biotin/streptavidin nanoparticles. The mechanism of action involves using glucose as the triggering mechanism for a final red fluorescent emission from the quantum dots. This device serves as a prototype for a wide variety of applications which includes other biosensor devices, lab-on a-chip devices, in-vivo drug delivery systems and “micron size” dispersible bio/chem sensors for environmental, military and homeland security applications.


Nanotech 2008 Conference Program Abstract