2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Cancer Cell Diagnosis by Targeted Delivery of Nanodevices

I. Hajdu, E. Nagy, M. Bodnar, J.F. Hartmann, S. Damjanovich, J. Kollar, G. Vamosi, J. Borbely
University of Debrecen, HU

Keywords:
targeted delivery, MRI contrast agent, nanodevices

Abstract:
Cancer Cell Diagnosis by Targeted Delivery of Nanodevices I. Hajdu1, E. Nagy1, M. Bodnar1, J.F. Hartmann2, S. Damjanovich1, J. Kollar1, G. Vamosi1, and J. Borbely1,3 1University of Debrecen, H-4010 Debrecen, Hungary 2Elizanor LLC, Princeton Junction, NJ 08550, USA 3BBS Nanotechnology Ltd., H-4225 Debrecen, Hungary Abstract Currently macromolecular contrast agents for magnetic resonance imaging (MRI) are evaluated. Biomolecules and their modified derivatives may form sable complexes with paramagnetic ions, thus increasing the molecular relaxivity of carriers. In this work synthesis of selective biomolecules based nanodevices (SBND) for targeted delivery of MRI contrast agents are described. Nanoparticles (NPs) have been constructed by self-assembling of chitosan (CHIT) as polycation and poly-gamma glutamic acid (PGA) as polyanion. NPs formed are able to uptake Gd-ion having suitable molecular relaxivity. Folic acid (FA) was linked to the NPs to produce NP-FA bioconjugates that were used for targeted in vitro delivery to human cancer cell line. Confocal microscopic imaging confirmed the receptor mediated uptake of NP-FA-Gd conjugates into the HeLa cells, which overexpress the folate receptor. A specific uptake has been observed for NP-FA-Gd nanodevices vs. NP-Gd particles lacking FA moiety. References I. Hajdu, M. Bodnar, G. Filipcsei, J. F. Hartmann, L. Daroczi, M. Zrinyi, J. Borbely: Colloid Polym. Sci., 2007. X. Wen, E.F. Jackson, R.E. Price, E.E. Kim, Q. Wu, S. Wallace, C. Charnsangavej, J.G. Gelovani, and C. Li: Bioconjugate Chem. 2004.


Nanotech 2008 Conference Program Abstract