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Using Microfluidics for Metabolic Monitoring of Single Embryo Cultures
T. Thorsen, J.-P. Urbanski, M.T. Johnson, D.D. Craig, D.L. Potter, D.K. Gardner
Massachusetts Institute of Technology, US
Keywords:
microfluidics, in-vitro fertilization, metabolism
Abstract:
In recent years, microfluidics has demonstrated novel methods of culturing and studying cells. Within well-defined volumes comparable to the sample of interest, media, nutrients or toxins may be precisely targeted to individual cells via diffusion or advection, enabled by integrated valves and pumps. Tools that both enable a repeatable and systematic means of single embryo culture, as well as provide information on metabolic activity of individual embryos, would be of significant utility to the embryologist. To address the need of controlled culture environments and improve embryo health and subsequent positive implantation rates, we have developed programmable microfluidic devices for embryo culture and quantifying metabolite levels in the culture media.
Nanotech 2008 Conference Program Abstract
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