SiRNA for Therapeutic Immuno-modulation: Simultaneous Delivery of Cytokine-targeted SiRNA and DNA Antigens to Dendritic Cells using Polymer Microcarriers

Keywords:
immunotherapy, cytokine, dendritic cells, vaccine, IL-10

Abstract:
RNA interference mediated gene knockdown is a potential immuno-modulatory tool for tuning dendritic cells (DC) activation and function in-vivo. We have developed a novel multi-tiered delivery system for combinatorial administration of SiRNA and plasmid DNA antigens in a single gene carrier system. Our hypothesis is that, co-delivery of IL-10 specific SiRNA along with DNA antigens to the same dendritic cells would inhibit IL-10 production, enhance DC activation, antigen presentation and T cell response. Non-toxic, polyethyleneimine (PEI)-functionalized, poly(lactic-co-glycolic acid) (PEI-PLGA) microparticles co-delivering IL-10 SiRNA and DNA antigen exhibited significantly higher (p

Nanotech 2008 Conference Program Abstract