2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

SiRNA for Therapeutic Immuno-modulation: Simultaneous Delivery of Cytokine-targeted SiRNA and DNA Antigens to Dendritic Cells using Polymer Microcarriers

A. Singh, H. Nie, B. Ghosn, K. Roy
The University of Texas at Austin, US

Keywords:
immunotherapy, cytokine, dendritic cells, vaccine, IL-10

Abstract:
RNA interference mediated gene knockdown is a potential immuno-modulatory tool for tuning dendritic cells (DC) activation and function in-vivo. We have developed a novel multi-tiered delivery system for combinatorial administration of SiRNA and plasmid DNA antigens in a single gene carrier system. Our hypothesis is that, co-delivery of IL-10 specific SiRNA along with DNA antigens to the same dendritic cells would inhibit IL-10 production, enhance DC activation, antigen presentation and T cell response. Non-toxic, polyethyleneimine (PEI)-functionalized, poly(lactic-co-glycolic acid) (PEI-PLGA) microparticles co-delivering IL-10 SiRNA and DNA antigen exhibited significantly higher (p


Nanotech 2008 Conference Program Abstract