2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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Clean Technology 2008

In-vitro evaluation of carbon nanotube mediated gene delivery for colon cancer

A. Kulamarva, M. Malhotra, J. Bhathena, S. Sebak, S. Prakash
McGill University, CA

Keywords:
colorectal cancer, apc gene, carbon nanotubes, gene delivery. targeted delivery, cytotoxicity

Abstract:
Colorectal cancer is the second most common cause of cancer related deaths in North America. Mutations in DNA, specifically in the APC gene are an early event in tumor development, and are detected in a majority of colorectal tumors. We have investigated the potential of carbon nanotubes to deliver the non-mutated APC gene to colorectal cancer cell lines to act as a gene replacement therapy. Single walled carbon nanotubes were covalently functionalized with the wild type gene for cell transfection, using SW480 colon carcinoma cells which are known to express truncated APC. Nanotube-DNA complexes were analyzed by transmission electron microscopy and agarose gel shift assay. The transfection efficiency of carbon nanotubes was evaluated by fluorescence microscopy and flow cytometry. The presence and expression of wild type APC gene in the cell lines was confirmed by western blot analysis. The results indicate that carbon nanotubes can be successfully used to deliver plasmid containing APC gene into colon cancer cell lines and offer a viable method for gene delivery applications.


Nanotech 2008 Conference Program Abstract