2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

A Co-Culture Model for Nanotoxicity and Tissue Engineering Studies

Y.-H. Wang, S. Jandhyam, V.V. Dukhande, W.J. Gao, H.-Y. Gu, M.B. Lai, S.W. Leung, J.C.K. Lai
Idaho State University College of Pharmacy & Biomedical Research Institute, US

Keywords:
co-culture model, tissue engineering, nanotoxicity, cell signaling

Abstract:
Cell model systems in vitro play vital roles in recent advances in toxicological and tissue engineering research. They are particularly versatile in facilitating signaling and related mechanistic studies. Although the 3-D cell models better simulate the in vivo cellular architecture in tissues and organs, the advances made with such models were largely based on studies employing only a single cell type. Thus, there is a need for developing more co-culture cell systems because they provide different structural and functional perspectives that single-cell-type models do not offer. Our work on cerebrocortical neurons co-cultured on a monolayer of astrocytes reveals that neurons are more susceptible to manganese toxicity than astrocytes even though the astrocytes appear to provide some protective effect on the neurons in co-culture. We have adopted a similar approach to develop a co-culture cell model for short- and longer-term nanotoxicity and tissue engineering studies. Our co-culture model consists of a monolayer of human astrocytoma U87 (astrocytes-like) cells onto which we seed human neuroblastoma SK-N-SH (neurons-like) cells. We have characterized and optimized the conditions whereby these two cell types could be co-cultured and have employed this co-culture model to further elucidate the cytotoxicity of metallic oxide nanoparticles.


Nanotech 2008 Conference Program Abstract