Non-Condensing Calcium Alginate Microspheres for Macrophage-Selective Gene Delivery and Transfection
S. Jain, M. Amiji
Northeastern University, US
non-condensing polymer, gene delivery, macrophages
The main objective of the study was to design a non-condensing polymer microsphere-based gene delivery system for macrophages. An external gelation technique was used to prepare plasmid DNA-encapsulated calcium alginate microspheres. The microspheres were characterized for particle size, surface morphology, and DNA loading efficiency. Stability of encapsulated DNA was assessed by agarose gel electrophoresis. Qualitative and quantitative transfection efficiency of beta-galactosidase ( -gal) and interleukin-10 (IL-10) encoding plasmid DNA was determined upon administration to P388D1 murine alveolar macrophages. The results indicated that the calcium-alginate microspheres formed via external gelation technique were spherical in shape with an average diameter of approximately 1.0 µm. Plasmid DNA was optimally encapsulated with an efficiency of 50% and remained intact during the formulation process. Murine macrophages efficiently internalized the microspheres as assessed by fluorescence microscopy. Additionally, high transfection efficiency of -gal and IL-10 encoding DNA was observed with the microsphere delivery system relative to control. The results of this study show that non-condensing calcium alginate microspheres can be formulated for macrophage-specific delivery and afford high transfection in these cells.
Nanotech 2008 Conference Program Abstract