Lincoln University, US
Erythrocytes, Red Blood Cells (RBCs), have been used as bioreactors and drug delivery vehicles. They are considered biocompatible carrier. RBCs are also used as biological carriers for enzymes and peptides. Hypo-osmotic dialysis technique is the most commonly employed to achieve drug encapsulation in RBCs. However, the use of RBCs as carriers for fluorescence labeled protein has not been exploited. We have been investigating the feasibility of utilizing RBCs as optical carriers. The first step is to ensure proper encapsulation and signal capture of the fluorescent probes. Streptavidin labeled with Alexa Fluor 750 (SA-AF 750) was utilized as the probe which was then loaded into the RBCs by using the hypo-osmotic dialysis technique. The loaded cells were scanned by using a spectrometer. The excitation scan was set at 750nm. The results showed a peak signal at 775 nm which was an indication of presence of AF 750 in the RBC’s. This model could be used in future work to design in vivo optical sensors and in vivo imaging.
Nanotech 2008 Conference Program Abstract