2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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Clean Technology 2008

Chitosan Nanoparticle Combined with Ultrasound for Cervical Cancer Detection

K-C Tsai, M-J Shieh, S-J Yang, W-L Lin, W-S Chen
Institute of Biomedical Engineering, TW

Keywords:
photodynamic medicine, 5-Aminolevulinic acid, chitosan, nanoparticle, ultrasound

Abstract:
Photodynamic medicine is a novel approach for cancer detection and treatment via different photosensitizers and suitable light source. The 5-Aminolevulinic acid (5-ALA) can be converted into protoporphyrin IX (PpIX) that can be utilized as a fluorescent probe for tumor detection. However, the fluorescent quantity of PpIX sometimes is low for detection. In this study, 5-ALA was encapsulated in chitosan nanoparticles, and then HeLa cancer cells uptake was enhanced by ultrasound to increase the fluorescent quantity of PpIX. We mixed chitosan with 5-ALA to prepare chitosan nanoparticles loaded with 5-ALA (CNA) by ionic gelation method. The size and zeta potential of CNA were measured by transmission electron microscope and zetasizer, respectively. The size of CNA was 102.4 nm and the zeta potential was 25.0 mV that was enough to prevent particles from aggregating. The loading efficiency of 5-ALA in CNA was up to 90%. Bioassy results of CNA for HeLa cell was done, and the result revealed that HeLa cell could uptake CNA and the amounts of CNA uptaken by cell could be enhanced greatly by ultrasound. According to this concept, we designed a novel photodynamic detection system to enhance the accuracy of diagnosis for early cervical cancer.


Nanotech 2008 Conference Program Abstract