2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Silicon Dioxide Nanoparticles Exert Dissimilar Cytotoxic Effects on Mammalian Cell Types

S. Jandhyam, M.B. Lai, V.V. Dukhande, A. Bhushan, C.K. Daniels, S.W. Leung, J.C.K. Lai
Idaho State University, US

Keywords:
silicon dioxide nanoparticles, nanotoxicity of nanoparticles, human cell types, cytotoxicity

Abstract:
Applications of nanomaterials (e.g., silicon dioxide (SiO2) nanoparticles) are ubiquitous. Thus, environmental and occupational exposure to these nanoparticles could pose potential health risk. SiO2 microparticles are toxic in animals in vivo and SiO2 nanoparticles are toxic in some mammalian cell types in vitro although studies using human cell types are scarce. Our goal is to systematically characterize the cytotoxic effects of SiO2 nanoparticles on various human cell types and elucidate the underlying mechanisms. In this study, we have compared the effects of the nanoparticles on human astrocytoma U-87 (astrocytes-like), human neuroblastoma SK-N-SH (neurons-like), and normal human fibroblasts. Results from MTT assay and cellular lactate dehydrogenase release suggest that treatment of the cell types with SiO2 nanoparticles (0.1-100 ug/ml) exerted dose-related, differential cytotoxic effects on the human cell types studied, the effect being least pronounced in fibroblasts. Observations of the treated cells using bright-field light microscopy were compatible with the results noted using MTT assay. We are investigating the cell signaling mechanism underlying cell death induced by SiO2 nanoparticles. Thus, our results indicate SiO2 nanoparticles exert differential cytotoxic effect on human cell types and may have implications in risk assessment of environmental and occupational exposure to these nanoparticles. (Our studies were supported by an USAMRMC Project Grant (Contract #W81XWH-07-2-0078) and NIH Grant #P20 RR016454 from the Idaho INBRE Program of the National Center for Research Resources.)


Nanotech 2008 Conference Program Abstract