2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

In vitro transfection efficiency of chitosan nanoparticles for gene delivery

M. Malhotra, A. Kulamarva, S. Sebak, J. Bhathena, S. Prakash
McGill University, CA

Keywords:
nanoparticles, gene delivery, neuronal cells

Abstract:
Cationic nanopolymer mediated transfection using Chitosan has been first described as an efficient delivery system for plasmids by Mumper et al. (1995). Due to the cationic charges, biocompatibility and biodegradability, chitosans seems to be a potential gene delivery system. In our study we aim to develop surface functionalized chitosan nanoparticles of range less than 100nm for gene delivery, exhibition an efficient active targeting, reduced immunogenicity and cytotoxicity. The chitosan nanoparticles were prepared using an ionic gelation scheme, which involves interaction of positively charged chitosan with negatively charged sodium tripolyphosphate (TPP). We determined the effect of change in pH on particle size and surface charge. Gene loading efficiency of chitosan nanoparticles were performed at varying weight ratios of chitosan:gene. The maximum gene loading efficiency obtained was approximately 92% as determined by UV spectrophotometer. The transfection efficieny of gene loaded chitosan nanoparticles was evaluated in vitro on neuronal cells and were also analyzed for cytotoxicity using an MTS cytrotoxicity assay. The nanoplexes resulted in significant transfection but were highly toxic. We look forward to enhance the efficacy of the delivery system by surface coating them with a hydrophilic molecule like PEG that will enhance cellular uptake and reduce cellular toxicity.


Nanotech 2008 Conference Program Abstract