2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

Phage-derived Vehicles for Gene and Drug Delivery

V. Petrenko
Auburn University, US

Keywords:
phage-derived vehicles, gene, drug delivery

Abstract:
Phage-based bioselective nanoparticles specific for cancer and bacterial cells were exploited as molecular recognition interfaces in drug- and gene-delivery systems. The phage were selected from the multibillion landscape phage libraries and converted to the recognition interface—the cell-specific peptides fused to the major coat protein—using intrinsic structural duplicity of the phage proteins. In contrast to poorly controllable peptide- and antibody-conjugation procedures, the new landscape phage-based approach relies on very powerful and extremely precise mechanisms of selection, biosynthesis and self assembly. No reengineering of the selected phage is required at all: the phages themselves serve as the source of the final product—coat protein genetically fused to the targeting peptide. The major coat protein constitute 98% of the total protein mass of the virion—the purity hardly reachable in normal synthetic and bioengineering procedures. In contrast to immunization procedure, the phage selection protocol may require tiny amounts of a target material for obtaining the specific phage ligands, affinity and selectivity of which may be controlled, if necessary, by exploring well developed depletion and affinity maturation procedures. Details of the approach will be discussed in the report.


Nanotech 2008 Conference Program Abstract