2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Multiplexed Proteolytic Enzyme Assay Based on Energy Transfer between Quantum Dots and Gold Nanoparticles

Y.H. Oh, Y.P. Kim, H.S. Kim
Korea Abvanced Institute of science and Technology, KR

Keywords:
chip-base, multiplexing, quantum dot, gold nanoparticle

Abstract:
Proteases are key enzymes that regulate both normal and malignant tumor progress. Evaluating these enzymes and their inhibitors, therefore, has a great potential to diagnostics or therapeutic treatment against specific diseases or disorders. We have demonstrated that enzyme protease can be effectively detected by using an energy transfer between quantum dot (QDs) and gold nanoparticles (AuNPs) on a chip surface. By applying the peptide-conjugated AuNPs to multiple QD donors immobilized on the glass surface, the assay of various proteases (matrix proteinase, caspase, and thrombin) will provide a great potential for analysis of enzyme proteases and their inhibitors with high specificity and sensitivity in a high-throughput manner. With different functionalities on multiple QD donors, energy transfer-based assay on a solid surface will provide the great potential for multiplexing as well as high-throughput assay and will be expected to be a powerful diagnostic tool to address many other interactions.


Nanotech 2008 Conference Program Abstract