2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

Imprinting soft biological gels down to one micron feature size

P. Agrawal, C. Hurth, N. Iyer, J. Gu, J. Yang, F. Zenhausern
Arizona State University, US

Keywords:
biological gels, biomaterials, 3D cell culture

Abstract:
Imprinting soft biological gels is a unique way to create spatially complex three dimensional structured microenvironments for cell culture. These structures have the potential to be used as engineered biological tissues and could have significant effects on many biological processes. Indeed, it has been shown, by micropatterning of extracellular matrix gel, that morphogenesis can be controlled by tissue geometry that defines local cellular microenvironment. Most previous works on patterning of soft gels, however, focused on a pattern size larger than 50 µm. The imprinted gel structures were also not well characterized. In this work, we report our study of imprinting soft biological gels down to ~ 1µm using soft lithography techniques. Different gels, e.g. agarose, MatrigelTM, and peptide-based self-assembled PuramatrixTM have been studied. Stamp features with sloped and vertical sidewalls have been used. The replication of the stamp structure in the gels is characterized by atomic force microscope. The dependence on stamp feature shapes, aspect ratios, gel concentration etc will be presented. Finally, cell culture on micropatterned gel substrate will be discussed.


Nanotech 2008 Conference Program Abstract