Permeability of An In Vitro Model of Blood Brain Barrier (BBB)
COMSATS Institute of Information Technology, PK
drug delivery, in vitro modeling
Neurological disorders include a large variety of brain diseases which may be treated with drugs. Unfortunately, the majority of drugs under a certain development for neurological purposes are not crossing the Blood Brain Barrier (BBB). To evaluate pharmacokinetics property of neurological drugs mainly animal studies are performed, which render the development of drugs a long high cost process. Therefore, there is a need to develop an in vitro BBB model to measure permeability of novel drugs which are under research and development. From studies thrombin is known for decreasing permeability of endothelial cells. Some research groups have also worked on thrombin using epithelial cells. An In Vitro Model of BBB was develop and characterized involving endothelial cells or epithelial cells (MDCK). And then a novel approach was adopted to see the effect of thrombin on the permeability of the monolayer on transwel cell culture insert. Later, the results gotten from TEER measurement and Cell Permeability analyzer were measured.
Nanotech 2008 Conference Program Abstract