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Quantum dot (Qdot) labeling of gene expression analysis and single molecule imaging in neurons
A. Jeromin, Z. Riley, E. Byrnes, S. Parry, S. Smith, N. Dee, A. Jones
Allen Institute for Brain Science, US
Keywords:
quantum dot, Qdot, gene expression, labeling, in situ, hybridization
Abstract:
The Allen Brain Atlas1 is the first large scale atlas of gene expression in the mouse brain, using chromogenic in situ hybridization (cISH) of over 20,000 genes. Qdots have excellent potential for muliplex imaging of gene expression due to their photstability and tenability. In addition, they can be conjugated to a variety of ligands for labeling. We will report on our progress to develop a high-throughput multiplex gene expression platform in brain, using riboprobes and quantum dots conjugated to various ligands. The signal intensity obtained with these probes is comparable to cISH and will allow to correlate the spatial expression pattern of genes. We will show an example, using Qdots for single-molecule imaging of ion channels in the membrane of living neurons.
Nanotech 2008 Conference Program Abstract
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