Engineering and Evaluation of Carrier-Free Methotrexate Nanospheres
E. Hassan, S. Gumudavelli
Pharmaceutics International, US
solid drug nanospheres, nanopartilces, molecular coating, cancer, Methotrexate
Carrier-free methotrexate (MTX) nanospheres were manufactured using critical emulsion technique. The surface charge of the solid drug nanospheres (SDN) was modified by single or multiple molecular coating with various cationic and anionic polymers. Molecular coating with small molecules such as folic acid and vitamin B12 was also performed. Dissolution of SDN in aqueous pH 4 medium was performed using an ultra-centrifugal dialysis device. SDN were also subjected to thermal analysis using DSC. SDN had an average particle size range of 90-250nm. The negatively charged uncoated SDN (-40.0 Mv) acquired a positive surface charge of +34.0 Mv upon coating with chitosan. SDN had up to 6 folds higher dissolution rate than MTX raw material. SDN had twice the solubility value of MTX raw material after 24 hours of mixing. The increases in both dissolution and solubility reflect the modified physical properties of MTX SDN which were also verified form the significant change in melting point of SDN relative to MTX raw material.
Nanotech 2008 Conference Program Abstract