2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

Nanomolar range of nitric oxide and its bioimaging by NO amplifier coupled biosensor

R. Sharma, S. Kwon
Utah State University, US

Keywords:
nitric oxide, bioimaging, hypoxia, NO biosensor, NO amplifier, chelotropic traps

Abstract:
Nitric oxide (NO) is a small uncharged free radical abundant in nanomolar quantities that is involved in diverse physiological and pathophysiological mechanisms. NO is generated by three isoforms of NO synthase, endothelial, neuronal, and inducible ones. When generated in vascular endothelial cells, NO plays a key role in vascular tone regulation, in particular. Diaminofluorescein, diaminorhodamine, 1,2-Diaminoanthraquinone, fluorescent nitric oxide cheletropic traps are current emerging choices in nitric oxide imaging but all these have pitfalls of causing neurotoxicity. Here, we describe an amplifier-coupled fluorescent indicator for NO to visualize physiological nanomolar dynamics of NO in living cells (detection limit of 0.1 nM). An amplifier-coupled fluorescent indicator visualizes NO in single living cells. Its domain structures are sGC, sGC, CGY, sGC -CGY, and sGC -CGY. This genetically encoded high-sensitive indicator revealed that 1 nM of NO was enough to relax blood vessels. The nanomolar range of basal endothelial NO thus revealed appears to be fundamental to vascular homeostasis.


Nanotech 2008 Conference Program Abstract