2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

SNR Optimization for Microfabricated DNA Thermosequencing Platform

H. Esfandyarpour, R.F.W. Pease, R.W. Davis
Stanford University, US

Keywords:
thermosequencing, DNA, microfluidic, MEMS, SNR

Abstract:
Sanger DNA Sequencing method has been the primary DNA sequencing technology for the past three decades [1, 2]; however, inherent limitations in cost, speed and complexity have limited its usage in personalized medicine and ecological studies. A new technology called “Thermosequencing” can potentially reduce both the cost and complexity of DNA sequencing by using a microfluidic platform [2-5, 12]. We study enzymatic and heat profile modeling for Thermosequencing biosensor and discuss the real time simulation results for chemical concentrations involved in the detection system. We optimized the speed and signal to noise ratio of the sensor simultaneously. The modeling simulates not only the heat released through the DNA polymerization reaction and its corresponding temperature change, but also the heat and sensing efficiencies of different geometries and platform structures. The thermodynamic effects of a DNA coated bead are investigated. Then, based on heat diffusion results, we recommend a modified gated structure for the microfluidic detection platform by using control valves and show how this new platform could dramatically improve the detection efficiency. Finally, we discuss potential breakthrough advantages of DNA Thermosequencing in future in terms of cost, speed and efficiency.


Nanotech 2008 Conference Program Abstract